我们都爱笑tfboys】【铁血道光】【杭天琪近况】【致命伴侣电视剧】【恶鬼为夫】【神将世界韩当技能】【血刃百度影音】【田紫紫背景】【发动机吸入孔明灯】【穿越之魔法女皇】【方块射手】【大海热线主题曲】【王艇隆鼻】【幽灵鬼屋历险记】【qq三国水之精华】【鹤山haobc vip】【成濑理沙ed2k】【温翠屏】【我的白领大小姐】【别用下辈子来安慰我】【大庆资源与评价答案】【湖南警察持枪杀人案】【凌海电力商城刘秋彤】【心云简谱】【科学怪鱼国语】【黑道奶爸国语版】【职来职往英国专场】【特猫肉系是什么意思】【幻想神域商城待领区】【太剑玄天鸿蒙大道】【林海峰营养早餐】【官术 快眼看书】【一不留神演员表】【女欢女爱第四季

捕鱼游戏厅

您好,欢迎光临安诺伦(捕鱼游戏厅手机版)生物科技商城!
全国服务热线:400-965-8633
关注我们
  • 会员中心
  • 会员中心
    询价列表
    0

    最新加入的商品

    0 件商品 合计:¥ 0
  • 会员中心
    购物车

    最新加入的商品

    件商品 合计:¥ 0

全部产品分类

自主品牌
当前位置:首页 > DNA、RNA、PCR、文库 > 基因克隆 > RNA转录试剂盒 > INCOGNITO T7 5mC- & -RNA Transcription Kit
品牌:
  • 所有品牌
  • A
  • B
  • C
  • D
  • E
  • F
  • G
  • H
  • I
  • J
  • K
  • L
  • M
  • N
  • O
  • P
  • Q
  • R
  • S
  • T
  • U
  • V
  • W
  • X
  • Z

INCOGNITO T7 5mC- & -RNA Transcription Kit

C-ICTMY110510
货号 货期 规格 / 价格 询价

INCOGNITO T7 5mC- & -RNA Transcription Kit

品牌

CELLSCRIPT

货号

C-ICTMY110510

产品分类

RNA转录试剂盒

研究领域

产品概述

Store at -20°C in a freezer without a defrost cycle. Do not store at -70°C

形式

The INCOGNITO T7 5mC- & -RNA Transcription Kit* is optimized for high-yield synthesis of 5-methylcytosine- pseudouridine-containing RNA (5mC -RNA) by in vitro transcription (IVT) of a DNA templatethat has a properly-oriented phage T7 RNA polymerase promoter using T7-Scribe RNA polymerase,the canonical nucleotides ATP & GTP, and the modified nucleotides 5-methylcytosine-5'-triphosphate(5mCTP) & pseudouridine-5'-triphosphate ( TP). Although yield varies with the DNA template and otherfactors, a standard 20 μl IVT reaction using 1 μg of a control template DNA yields approximately 100 μgof 5mC -RNA in 2 hours at 37°C. The 5mC -RNA can be enzymatically 5'-capped and 3'-poly(A)-tailedusing CELLSCRIPT's ScriptCap m7G Capping System, ScriptCap 2'-O-Methyltransferase andA-Plus Poly(A) Polymerase to generate 5mC -mRNA for expression in cells. It has been shown that 5mC -mRNAs are translated into protein at higher levels and induce lower innateimmune responses in human and other mammalian cells that express various RNA sensors compared tocorresponding unmodified mRNAs.

试剂准备

RNase-Free DNase I is provided in a 50% glycerol solution containing 50 mM Tris-HCl, pH 7.5,10 mM CaCl2, 10 mM MgCl2 and 0.1% Triton X-100. ScriptGuard RNase Inhibitor is supplied in a50% glycerol solution containing 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 10 mM DTT, 0.1 mM EDTAand 0.1% Triton X-100. All other enzymes are provided in a 50% glycerol solution containing 50 mMTris-HCl, pH 7.5, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA and 0.1% Triton X-100.

产品描述

Unit Definition One unit of RNase-Free DNase I digests 1 g of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C. One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A. Functional Testing The INCOGNITO T7 5mC- & Ψ-RNA Transcription Kit is functionally tested under standard reactionconditions using the T7 Control Template DNA. The kit must produce at least 90 g of RNA from1 g of the T7 Control Template DNA in 2 hours at 37°C. Contaminating Activity Assays All components of the INCOGNITO T7 5mC- & Ψ-RNA Transcription Kit are free of detectable RNaseand DNase activity, except for the inherent activity of the RNase-Free DNase I component.

官网链接

数量

相关产品

选择 品牌 货号 产品名称 规格 分类 研究领域 说明书 数量 目录价
1 CELLSCRIPT C-MSC11610 T7 mScript Standard mRNA Production System 10 reactions RNA转录试剂盒
¥5495.00 订购 询价
2 CELLSCRIPT C-SRI6310K ScriptGuard RNase Inhibitor 10,000 units RNA转录试剂盒
¥4778.00 订购 询价
3 CELLSCRIPT C-MMA60710 MessageMAX T7 ARCA-Capped Message 10 reactions RNA转录试剂盒
¥5548.00 订购 询价
4 CELLSCRIPT C-CC15011H Cap-Clip Acid Pyrophosphatase 100 units RNA转录试剂盒
¥9625.00 订购 询价
5 CELLSCRIPT C-ACM04037 AmpliCap-Max T7 High Yield Message Maker Kit 25 reactions RNA转录试剂盒
¥6318.00 订购 询价
6 CELLSCRIPT C-MSC100625 T7 mScript Standard mRNA Production System 25 reactions RNA转录试剂盒
¥12040.00 订购 询价

产品添加成功!

购物车已有 0 件产品

加入询价单成功!

询价单已有 0 件产品

sitemap.xml